Bio-One franchise
A cleanup business providing biohazard removal, disinfection and related restoration services. At the end of 2025 there were 147 Bio-One locations in the US, 144 franchised and 3 company-owned.
- Cost to open
- $135K–$221K
- Franchise fee
- $60,000
- Royalty
- 7.5%; minimum applies
- Locations, 2025
- 1470 in 3 yrs
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Company snapshot
- SBA loans
- On the SBA Franchise Directory
From RINGSIDE DEVELOPMENT COMPANY D/B/A BIO-ONE COLORADO, INC’s 2026 filing; SBA status from the SBA Franchise Directory, October 28, 2026.
How much a Bio-One franchise costs
Opening one Bio-One costs an estimated $134,645 to $221,095. That covers the franchise fee and everything else needed to open, through the first months of working capital.
Source: RINGSIDE DEVELOPMENT COMPANY D/B/A BIO-ONE COLORADO, INC, 2026 filing, page 18
Bio-One franchise fee
The franchise fee is $60,000. You pay it when you sign the franchise agreement.
Source: RINGSIDE DEVELOPMENT COMPANY D/B/A BIO-ONE COLORADO, INC, 2026 filing, page 12
Royalty and ongoing fees
| Royalty | 7.5% of gross sales or the applicable minimum royalty, whichever is greater. |
|---|
The typical franchise royalty is 6% of sales, plus 2% for marketing. See franchise fees and royalties to compare.
Source: RINGSIDE DEVELOPMENT COMPANY D/B/A BIO-ONE COLORADO, INC, 2026 filing, page 13
Sales and earnings
Shares figures Bio-One publishes sales or earnings figures for its locations. The full tables, with notes on which locations are counted, are in its filing.
Source: RINGSIDE DEVELOPMENT COMPANY D/B/A BIO-ONE COLORADO, INC, 2026 filing, page 37
Locations opened and closed
| Year | Franchised | Company-owned | Closed |
|---|---|---|---|
| 2023 | 146 (+2) | 0 | 16 |
| 2024 | 152 (+6) | 0 | 15 |
| 2025 | 144 (−8) | 3 | 20 |
Closed or left counts terminations, non-renewals, locations bought back by the franchisor and locations that stopped operating.
See which brands grew most in the fastest-growing franchises rankings.
Bio-One passes four of the five tests on our list of the best franchises to own; more of its franchised locations closed or left than is typical.
Source: RINGSIDE DEVELOPMENT COMPANY D/B/A BIO-ONE COLORADO, INC, 2026 filing, page 39
How Bio-One compares
Of the 29 restoration franchises on this site, Bio-One is the 10th cheapest to open and the 10th largest by number of locations.
| Measure | Bio-One | Typical restoration |
|---|---|---|
| Cost to open, low end | $135K | $157K |
| Royalty | 7.5% | 7% |
| Closed or left per year | 11.5% | 6.5% |
| Change in franchised locations, 3 yrs | 0% | +8.1% |
| Shares earnings figures | Yes | 25 of 29 |
Typical is the median across the 29 brands in restoration franchises.
